What is the theory behind ion exchange chromatography?

Ion exchange (IEX) chromatography is a technique that is commonly used in biomolecule purification. It involves the separation of molecules on the basis of their charge. In anion exchange the stationary phase is positively charged whilst in cation exchange it is negatively charged.

How does ion chromatography work?

How Does Ion Chromatography Work? Ion chromatography, a form of liquid chromatography, measures concentrations of ionic species by separating them based on their interaction with a resin. As an ion extraction liquid, known as eluent, runs through the column, the absorbed ions begin separating from the column.

How do ion suppressors work?

The suppressor removes sodium ions in the eluent and changes sodium carbonate to carbonic acid with lower conductivity, allowing the background levels to decrease. In addition, the suppressor changes sodium ions (cations), to hydrogen ions with higher conductivity.

Is a cation positively charged?

Positively charged ions are called cations; negatively charged ions, anions.

What are the disadvantages of ion exchange chromatography?

One of the main disadvantages of ion exchange chromatography is its buffer requirement: because binding to IEX resins is dependent on electrostatic interactions between proteins of interest and the stationary phase, IEX columns must be loaded in low-salt buffers.

Is Ion Chromatography HPLC?

Ion chromatography is a modified version of HPLC with a capacity for precise and highly sensitive detection of inorganic ions in a complex matrix. Ion chromatography has instrumental configurations similar to those of HPLC: a pumping system, a column compartment, and a detecting system.

What is ion chromatography test?

Ion Chromatography (IC) analysis. Ion chromatography (IC) laboratories support analytical testing projects requiring separation and identification of components and trace materials. Ion chromatography is a form of liquid chromatography, using ion-exchange resins to separate atomic and molecular ions for analysis.

What type of detector is used in ion chromatography?

Conductivity detection is the standard detection method in ion chromatography. It is used to detect a wide range of analytes ranging from anions to cations to amines. The Metrohm conductivity detector “sees” all ionic components, making it the most widely used detector.

How accurate is ion chromatography?

The accuracy and precision of an IC system constructed from commercially available components was tested in the concentration range 10–5000 μg l−1 for chloride, nitrate and sulphate. At a concentration of 200 μg l−1 of these components the accuracy was 2% or better.

Which type of chromatography is ion exchange chromatography?

adsorption chromatography
Ion exchange chromatography is a type of adsorption chromatography so that, charged molecules adsorb to ion exchangers reversibly so, the molecules can be bounded or eluted by changing the ionic environment.

Is Ion Chromatography destructive?

The method is non-destructive, because samples can be taken directly from aqueous solutions.