What is a half log dilution?

A tenfold dilution for each step is called a logarithmic dilution or log-dilution, a 3.16-fold (100.5-fold) dilution is called a half-logarithmic dilution or half-log dilution, and a 1.78-fold (100.25-fold) dilution is called a quarter-logarithmic dilution or quarter-log dilution.

What is the formula of dilution factor?

The required dilution factor can then be calculated as: = original concentration ÷ target concentration.

How do you calculate step dilution?

In serial dilutions, you multiply the dilution factors for each step. The dilution factor or the dilution is the initial volume divided by the final volume. For example, if you add a 1 mL sample to 9 mL of diluent to get 10 mL of solution, DF=ViVf = 1mL10mL=110 .

What is a 2 fold dilution?

A two-fold dilution reduces the concentration of a solution by a factor of two that is reduces the original concentration by one half. A series of two-fold dilutions is described as two-fold serial dilutions.

What is the final dilution of a 50 ml diluent when a 10 ml sample is added to it?

here for the answer, The explanation. When 10 ml sample is added to 50 ml dilute, then the total volume of the Diluted sample would be 60 ml.

How do you do a 1 50 dilution?

Explanation: If you want to make a 1/50 dilution you add 1 volume part of the one to 49 parts of the other, to make up 50 parts in all.

How do you do a 5x dilution?

How much initial sample and diluent should you use? Answer: 1:5 dilution = 1/5 dilution = 1 part sample and 4 parts diluent in a total of 5 parts. If you need 10 ml, final volume, then you need 1/5 of 10 ml = 2 ml sample. To bring this 2 ml sample up to a total volume of 10 ml, you must add 10 ml – 2 ml = 8 ml diluent.

What is a 3 fold dilution?

Since the dilution-fold is the same in each step, the dilutions are a geometric series (constant ratio between any adjacent dilutions). For example: 1/3, 1/9, 1/27, 1/81 Notice that each dilution is three-fold relative to the previous one. In four dilutions, we have covered a range of 181/3 = 60-fold.

What is a dilution factor and how to calculate it?

Dilution Factor is the factor by which the stock solution is diluted. It may be expressed as the ratio of the volume of the final diluted solution (V2) to the initial volume removed from the stock solution (V1), as shown in the equation above.

What is a 2-fold dilution?

As another example, a 2-fold dilution is the same as a dilution factor of 2. Therefore, dilution by any factor X is equivalent to X-fold dilution. Please note that the dilution factor calculator below uses the metric unit for volume (fL, pL, nL, μL, mL, and L).

What is a log dilution used for?

Dilution is the process of making a solution weaker or less concentrated. In microbiology, serial dilutions (log dilutions) are used to decrease a bacterial concentration to a required concentration for a specific test method, or to a concentration which is easier to count when plated to an agar plate.