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How do you do solid state fermentation?

How do you do solid state fermentation?

This process consists of depositing a solid culture substrate, such as rice or wheat bran, on flatbeds after seeding it with microorganisms; the substrate is then left in a temperature-controlled room for several days.

Are used for solid state fermentation?

Solid State Fermentation (SSF) Substrate The most regularly used solid substrates are cereal grains (rice, wheat, barley, and corn), legume seeds, wheat bran, lignocellulose materials such as straws, sawdust or wood shavings, and a wide range of plant and animal materials.

How is cellulase produced?

Cellulases are the enzymes that hydrolyze β -1,4 link- ages in cellulose chains. They are produced by fungi, bacteria, protozoans, plants, and animals. To hydrolyze and metabolize insoluble cellulose, the micro- organisms must secrete the cellulases (possibly except BG) that are either free or cell-surface-bound.

What is the problem in solid state fermentation?


No Solid State Fermentation Disadvantage
1 Engineering problems due to the build-up of temperature, pH control, oxygen transfer, mass and heat transfer, substrate and moisture gradients
2 Uneven distribution of the cell mass, nutrients, temperature, pH, moisture content

How do you prepare nutrient media for solid state fermentation?

1) you use an organic waste to produce citric acid in the SSF; 2) this material is rich with a carbon source but is limited in nitrogen; 3) to produce more citric acid you you need an abundant growth of fungus and readily available carbon source.

What are advantages of solid state fermentation?

Although less employed at an industrial level, SSF is recognized to commonly offer a series of advantages over submerged (or liquid) fermentation (SmF), such as higher yields and productivities, extended stability of products, lower production cost, lower protein breakdown (which is especially important if an enzyme is …

How do you make cellulase solution?

Add 60.0 g of glacial acetic acid (1.05 g/mL) to 800 mL of distilled water. Adjust the pH of this solution to pH 4.5 by the addition of 5 M (20 g/100 mL) NaOH solution. Adjust the volume to 1 litre. Stable at room temperature for several months.

What is solid state fermentation and submerged fermentation?

The key difference between solid state fermentation and submerged fermentation is that solid-state fermentation involves the cultivation of microorganisms on a solid substrate with a low moisture content while submerged fermentation involves the cultivation of microorganisms in a liquid medium which has more than 95% …

How the pH can be maintained during solid state fermentation?

Maintain the moisture in a Humidity chamber, using the minimal media (pH adjusted). The pH of 1 M solution of citric acid is 3.24 and the pH range for fungal growth is 4-5.8. Usually, during fermentation of carbohydrate substrates especially, acidic products are released and the pH of medium drops.

What are the significant disadvantages of solid state fermentation?

Disadvantages of Solid State Fermentation Ø Scale-up of the fermentation process is difficult. Ø Fermentation usually produces excessive heat in the medium. Ø The environmental conditions of the microbes cannot be regulated in SSF. Ø Bacterial contamination sometimes problematic.

Which is better solid state fermentation or submerged fermentation?

SSF is preferred over SmF as it is cost effective, eco-friendly and it delivers high yield of enzyme. SSF process utilizes agricultural and industrial wastes as solid substrate. The contamination level is substantially reduced in SSF through low moisture content.

How do you make cellulase enzymes from sago fronds?

Cellulase enzymes were produced through both solid-state and submerged cultivation of sago frond by Actinomicycetes. Two substrates, sago frond flour and pulp of sago fronds, were inoculated by isolate HJ4 (3b) and HJ4 (5b).

What is the best method for the production of cellulose?

Solid state fermentation for cellulase production. Successful utilization of cellulosic material as a renewable carbon source depends on the development of economically feasible process technologies for the production of cellulose. This enzyme has various industrial applications and is now considered as major group of industrial enzyme.

What is the function of cellulase?

Cellulase is the enzymatic system, which degrades cellulose chains to glucose monomers. Cellulase is a group of three enzymes endoglucanase, exoglucanases and β-glucosidases which act together to hydrolyze cellulose to glucose units. Cellulases are found in bacteria, fungi, plants, and some animals.