What are hek293f cells?

293-F cells are GIBCO brand cells. These are clonal isolates derived from transformed human embryonal kidney (HEK) cells. The 293-F strain is a fast-growing variant of the 293 cell line. They have been adapted to suspension culture in serum-free media, 293 SFM II.

What are freestyle cells?

Prepared from low-passage Master Cell Bank cultures derived from parental 293-F cells that were re-cloned by limiting dilution. The 293 clonal derived cultures are maintained in serum-free conditions for only 30 to 35 total passages.

What are ExpiCHO cells?

ExpiCHO-S™ Cells are derived from a non-engineered subclone that has been screened and isolated from CHO-S Chinese hamster ovary (CHO) cells (1). ExpiCHO-S Cells are maintained in suspension culture, have minimal tendency to clump, and grow to high density in ExpiCHO™ Expression Medium (Cat.

What have HEK293 cells been used for?

HEK 293 cells have been widely used in cell biology research for many years, because of their reliable growth and propensity for transfection. They are also used by the biotechnology industry to produce therapeutic proteins and viruses for gene therapy as well as safety testing for a vast array of chemicals.

What does HEK293 stand for?

Human embryonic kidney 293
Human embryonic kidney 293 (HEK293, HEK-293, or HEK) cells are one of the most common cell lines used for research purposes, second only to HeLa cells.

Why do we use CHO cells?

CHO cells became popular because they have a low chromosome number and were used to study genetics. These characteristics have made CHO cells the most important cell line for the production of therapeutic proteins including humanized antibodies.

Are HEK293 immortal?

Thus, HEK293 is not a model for normal human cells; these cells are immortalized already by known oncogene but not malignant yet.

Where do HEK-293 cells come from?

HEK293 is a cell line derived from human embryonic kidney cells grown in tissue culture. They are also known, more informally, as HEK cells. This particular line was initiated by the transformation and culturing of normal HEK cells with sheared adenovirus 5 DNA.