What are the 5 methods of sterilization?

Top 5 Methods Used for Sterilization | Microbiology

• Method # 1. Moist Heat Sterilization:
• Method # 2. Dry Heat Sterilization:
• Method # 3. Gas Sterilization:
• Method # 4. Sterilization by Radiation:
• Method # 5. Sterilization by Filtration:

What are the methods of sterilization?

Other Sterilization Methods

• Ionizing Radiation.
• Dry-Heat Sterilizers.
• Liquid Chemicals.
• Performic Acid.
• Filtration.
• Microwave.
• Glass Bead “Sterilizer”
• Vaporized Hydrogen Peroxide (VHP®)

What is the function of sterilization?

Sterilization destroys all microorganisms on the surface of an article or in a fluid to prevent disease transmission associated with the use of that item.

What is sterilization explain physical methods of sterilization?

Sterilization, which is any process, physical or chemical, that destroys all forms of life, is used especially to destroy microorganisms, spores, and viruses. Precisely defined, sterilization is the complete destruction of all microorganisms by a suitable chemical agent or by heat, either wet steam…

What are the parts and functions of autoclave?

Critical Components of an Autoclave

• Vessel. The vessel is the main body of the autoclave and consists of an inner chamber and an outer jacket.
• Control System.
• Thermostatic Trap.
• Safety Valve.
• Waste-Water Cooling Mechanism.
• Vacuum System (if applicable)
• Steam Generator (if applicable)

What are the 2 types of sterilization?

There are two distinctive types of heat-based sterilization: Moist Heat Sterilization and Dry Heat Sterilization.

What is the best method of sterilization Why?

The laboratory sterilization method of choice in most labs is autoclaving: using pressurized steam to heat the material to be sterilized. This is a very effective method that kills all microbes, spores, and viruses, although, for some specific bugs, especially high temperatures or incubation times are required.

What is sterilization and its types?

Common methods of sterilization include physical methods and chemical methods. Physical methods include dry heat, steam, radiation, and plasmas. Radiation encompasses a variety of types, including gamma radiation, electron beam, X-ray, ultraviolet, microwave, and white (broad spectrum) light.

Which of the following are the methods of sterilization?

Physical method a)Dry heat sterilization: Eg: Incineration, Direct flame, Red heat, Hot air. b)Moist heat sterilization: Eg: Pasteurization, Tyndallisation,Autoclave. c)Sterilization by radiation: Eg: Use of Ultra-violet rays: UV light(Non-ionising), Ionising radiations: X-rays, Gamma rays, beta rays.

What is dry heat sterilization and what are its applications?

This principle is used in instruments like hot air oven and incineration, which generates very hot moisture-free air. The primary industrial application of dry heat sterilization is in the sterilization of glass bottles which are to be filled aseptically.

How does heat sterilization kill microbes?

Heat method is the most common method of sterilization that kills the microbes directly. Depending on the duration of heating and the temperature of heat, the effect of sterilization varies. When a substance is exposed to the direct flame for a long time, the microbe molecules shrink and die.

What temperature is used to sterilize thermostable products?

This method of sterilization is applicable to thermostable products. Still, it can be applied to both moisture-sensitive and moisture-resistant products, for which dry (160–180°C) and moist (121–134°C) heat sterilization procedures are respectively used.