What color is rhodamine B?

Rhodamine B

What wavelength does rhodamine B absorb?

Figure 4 shows the absorption and emission spectra of rhodamine B. It is clear that both absorption and emission vary very little with the solvent, with absorption maximum at around 550 nm and emission maximum at around 570 nm for all three measurements.

Is rhodamine B soluble in water?

Rhodamine B base is also used as a laser dye, tunable around 610 nm with the fluorescence yield being temperature dependent. Solubility : Soluble in ethanol (1 mg/ml), methanol, chloroform, and DMF. Insoluble in water.

What wavelength is rhodamine?

Rhodamine B is a fluorescent compound with an excitation peak at 546 nm and an emission peak at 568 nm.

Is rhodamine B hydrophilic or hydrophobic?

Fluorescent dye Rhodamine B was used as a model hydrophilic drug in controlled release experiments after it was encapsulated in solution-blown soy-protein-containing hydrophilic nanofibers as well as in electrospun hydrophobic poly(ethylene terephthalate) (PET)-containing nanofibers.

What is rhodamine B used for?

As one of the most commonly used dyes, Rhodamine B (RhB) is widely used in industrial purposes, such as printing and dyeing in textile, paper, paints, leathers etc. However, the organic dyes will cause serious environmental and biological problems, even capable to induce irritation to the skin, eyes.

Why is rhodamine B fluorescent?

General description. Rhodamine B is bright-red in color. Rhodamine B is a xanthene dye, which functions as a water tracer fluorescent. It is used as a staining fluorescent dye.

Is Rhodamine B hydrophilic or hydrophobic?

What is Rhodamine B used for?

Why is Rhodamine B fluorescent?

Is rhodamine B cationic or anionic?

In addition, other cationic dyes, like malachite green (MG) and rhodamine B (RhB), as well as anionic dyes, like indigo carmine (IC), acid fuchsine (AF), acid orange (AO), and fluorescein disodium salt (FD), are also tested in this work.

Why is rhodamine fluorescent?

They are the chromophores and fluorophores of rhodamine dyes responsible for the strong light absorption and fluorescence emission in the visible region. One advantage of these dyes over rhodamines is the absence of lactone form due to the absence of the carboxyphenyl moiety.

How do you prepare Rhodamine B solution?

An aqueous Rhodamine B solution at 8.6 mM (molar ratio: x 1 = 1.55 × 10 – 4) was prepared with pure water (NANOpure II., Bamstead; 18 ΜΩ/cm resistance). A 30-50 μl solution was injected into the space between the mica surfaces after characterizing the properties of the mica substrate.

How is rhodamine used in spectrophotometer?

Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with instruments called fluorometers or in this case, a homemade spectrometer. Rhodamine dyes are used extensively in biotechnology applications such as, fluorescence microscopy, flow cytometry, fluorescent correlation spectroscopy and ELISA.

What is the molecular weight of rhodamine B?

Rhodamine B is a green powder with a molecular weight of 479.02. It is sometimes also known as Basic Violet 10. Rhodamine B is a fluorescent compound with an excitation peak at 546 nm and an emission peak at 568 nm.

What is the excitation peak of rhodamine B?

Rhodamine B is a fluorescent compound with an excitation peak at 546 nm and an emission peak at 568 nm. It can be excited using a 561 nm laser paired with a 586/15 nm bandpass filter, a configuration that can be found, for example, in the BD FACSCelesta™.