What is a biacore 3000?

Biacore™ 3000 is a system for real-time biomolecular interaction analysis using surface plasmon resonance (SPR) technology. SPR is a non-invasive optical measuring technique which measures the mass concentration of biomolecules in close proximity to the specially prepared surface.

What is Biacore technology?

The Biacore platform monitors molecular interactions in real time by using a non-invasive label-free technology. This technology reacts to changes in the concentration of molecules at the sensor surface as molecules bind to or detach from the surface.

What is SPR band?

Surface plasmon resonance (SPR) is the collective oscillation of conduction band electrons that are in resonance with the oscillating electric field of incident light, which will produce energetic plasmonic electrons through non-radiative excitation.

How much does Biacore cost?

The existing Biacore systems with similar features, like Biacore T100 or Biacore T200 systems, cost around $370K. In addition, Biacore dextrans chips cost $150 per chip.

Who owns Biacore?

GE Healthcare Limited
Biacore/Parent organizations

How is SPR calculated?

To measure the SPR angle, the change in the intensity of the reflected light with the angle of incidence is monitored. The angle of incidence is changed by rotating the turntable connected to an angular displacement transducer (Figure 3). The intensity of the reflected light is measured by a silicon solar cell.

How is SPR performed?

SPR occurs when polarized light strikes an electrically conducting surface at the interface between two media. This generates electron charge density waves called plasmons, reducing the intensity of reflected light at a specific angle known as the resonance angle, in proportion to the mass on a sensor surface.

How does SPR work?

How much does a Biacore cost?

How much protein do you need for SPR?

Dilute sample in SPR running buffer (HBS-N or HBS-EP) to the desired concentration, starting with 10 μg/mL of total protein. Typical analyte concentrations are 10–250 μg/mL, depending on the analyte affinity.

What are the necessary conditions for SPR?

Surface Plasmon Resonance. Surface Plasmon Resonance (SPR) is a physical process that can occur when plane-polarized light hits a thin metal film under total internal reflection conditions (1).

What can SPR tell you?

Surface plasmon resonance (SPR) can be used to analyze both binding affinities and kinetic parameters between a ligand and an analyte. The resultant sensorgram can indicate different types of binding events as well as provide both ka and kd, which can be used to determine an equilibrium dissociation constant KD.