What is creatinine Jaffe?

Creatinine in serum or urine is determined by Jaffe’s reaction where creatinine produces quantitatively an orange color with picric acid in alkaline medium. An investigation was thought to be necessary to determine the optimum alkali concentration required in standard, PFF of serum and urine.

What is the difference between Jaffe and enzymatic creatinine?

Background: The Jaffe and enzymatic methods are the two most common methods for measuring serum creatinine. The Jaffe method is less expensive than the enzymatic method but is also more susceptible to interferences. Interferences can lead to misdiagnosis but interferences may vary by patient population.

What is the Jaffe test?

The Jaffe reaction is a colorimetric method used in clinical chemistry to determine creatinine levels in blood and urine.

How does the Jaffe method work?

The method is based on the Jaffe reaction. Creatinine reacts with picrate ion formed in alkaline medium to develop a red-orange colour. The colour produced from the sample is then compared in a colorimeter at wavelength of 505 nm with that produced by a known amount of creatinine under the same condition.

What is the normal creatinine?

The typical range for serum creatinine is: For adult men, 0.74 to 1.35 mg/dL (65.4 to 119.3 micromoles/L) For adult women, 0.59 to 1.04 mg/dL (52.2 to 91.9 micromoles/L)

Why is creatinine important?

The creatinine blood test measures the level of creatinine in the blood. The result of this blood test is useful, as it is an important marker of how well the kidneys are working. Creatinine is the waste product of creatine, which the muscles use to make energy.

What is Enzymatic creatinine?

Creatinine amidohydrolase is used to measure serum creatinine in a totally enzymatic procedure. The amount of creatinine present is related to the rate of change in A340 and is determined from a standard curve. Absorbance and concentration are linearly related to 100 mg/liter and only 250 mul of serum is required.

Why is creatinine high in kidney failure?

Creatinine has been found to be a fairly reliable indicator of kidney function. Elevated creatinine level signifies impaired kidney function or kidney disease. As the kidneys become impaired for any reason, the creatinine level in the blood will rise due to poor clearance of creatinine by the kidneys.

Which method is best for creatinine test?

The Jaffe and enzymatic methods are the two most common methods for measuring serum creatinine. The Jaffe method is less expensive than the enzymatic method but is also more susceptible to interferences. Interferences can lead to misdiagnosis but interferences may vary by patient population.