What is sugarcane micropropagation?

Sugarcane micropropagation enables the identical production of the selected cultivars using PTC techniques. PTC refers to growing and differentiation of cells, tissues and organs isolated from the mother plant, on artificial semisolid or liquid media under aseptic and controlled conditions.

How are sugar cane plants propagated?

Sugarcane is propagated primarily by the planting of cuttings. The sections of the stalk of immature cane used for planting are known as seed cane, or cane sets, and have two or more buds (eyes), usually three. Seed cane is planted in well-worked fields.

What is in vitro culture plants?

Tissue culture is the in vitro aseptic culture of cells, tissues, organs or whole plant under controlled nutritional and environmental conditions [1] often to produce the clones of plants. The controlled conditions provide the culture an environment conducive for their growth and multiplication.

What are steps of micropropagation?

The process of micropropagation can be divided into four stages:

• Initiation stage. A piece of plant tissue (called an explant) is (a) cut from the plant, (b) disinfested (removal of surface contaminants), and (c) placed on a medium.
• Multiplication stage.
• Rooting or preplant stage.
• Acclimatization.

What is micropropagation in plant?

Introduction. Micropropagation is the clonal propagation of plants in closed vessels under aseptic conditions. It is also possible to produce plants from cells and tissues through the formation of adventitious shoots and somatic embryos (see CELL, TISSUE AND ORGAN CULTURE | In Vitro Regeneration Techniques).

What is tissue culture in sugarcane?

Currently a production part of the sugarcane plantlets are produced by tissue culture. Plant tissue culture is a process by which parts of plants can be grown in vitro in sterile culture in a medium containing a carbon source, minerals, growth factor and growth regulators such as hormones.

What is meristem culture?

Meristem culture is defined as the tissue culture technique, which uses apical meristem with 1-3 leaf primordia to prepare clones of a plant by the vegetative propagation. This technique primarily involves the isolation of meristem by applying a V-Shape cut in the stem.

What vegetative propagation is a sugarcane?

cuttings
Propagation Sugarcane is vegetatively propagated by planting part of a mature cane called “setts”. Setts should be cut from carefully selected mature canes. A few days before the cuttings are taken, the end of the canes are removed to break the apical dominance of the cane and promote the breaking of buds.

What is a sugarcane plant?

Sugar cane (Saccharum) is a perennial grass crop that food processors use to make sugar. Also called “sugarcane” or “sugarcane plants,” this agricultural grass grows in tall stems with feathery flowers. Some processors use the castoff created by pressing the sugar cane, called bagasse, to power electrical equipment.