What is Vic in PCR?

Applied Biosystems TaqMan Gene Expression assays, VIC, are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (VIC) on the 5′ end and a minor groove binder (MGB) and nonfluorescent quencher (NFQ) on the 3′ end.

Is Hex and Vic the same?

HEX has Ex at 535 nm and Em at 556 nm; VIC has Ex at 538 nm and Em at 554 nm. No big difference between them. However, some scientists claimed HEX could cause trouble due to its lactone structure. However, both VIC and HEX have similar lactone structure.

What color is Vic dye?

VIC is a fluorescent dye that was originally developed by Applied Biosystems, but is now proprietary to Life Technologies. VIC has an absorbance maximum of 538 nm and an emission maximum of 554 nm, thus emitting in the green-yellow part of the visible spectrum.

What is Vic fluorescence?

VIC is a fluorescent dye that was originally developed by Applied Biosystems (now, Thermofisher). It is used to fluorescently label oligonucleotides at the 5′-end (for use as probes in a variety of real-time PCR, hybridization, and fluorescence-based genetic analysis applications).

What is TaqMan real-time PCR?

TaqMan PCR is a type of real-time PCR and uses a nucleic acid probe complementary to an internal segment of the target DNA. The probe is labeled with two fluorescent moieties. The emission spectrum of one overlaps the excitation spectrum of the other, resulting in “quenching” of the first fluorophore by the second.

Why is it called real-time PCR?

In order to robustly detect and quantify gene expression from small amounts of RNA, amplification of the gene transcript is necessary. This measurement is made after each amplification cycle, and this is the reason why this method is called real time PCR (that is, immediate or simultaneous PCR).

What is ROX dye?

ROX dye is an inert fluorescent dye that can be added as one of the components in a qPCR master mix. Unlike reporter dyes such as SYBR™ Green or FAM™ dye, the fluorescence of ROX dye is not affected by amplification of the PCR product.

What does Vic stand for in fluorescent dye?

Join ResearchGate to ask questions, get input, and advance your work. In fluorescent dye name “VIC”, an abbreviation ” VIC ” stands for ” victoria “. How and why? It has been developed after the modifications of Aequorea victoria Green Fluorescent Protein.

What is the highest efficiency fluorochromes?

FITC (Ex-Max 494 nm/Em-Max 520 nm): Fluorescein isothiocyanate – has a very high efficiency of energy transfer from absorbed to emitted light and is one of the most commonly used fluorochromes. However, FITC is highly sensitive to pH changes and photo bleaching.

What is a tandem conjugate fluorochrome?

Like other tandem conjugates, it is a combination of two fluorochromes where the excited fluorochrome is able to transfer its fluorescent energy to the second molecule, which then fluoresces at a different, longer wavelength.

What is the size of a quantum dot fluorophore?

Fluorescence particles are not considered fluorophores ( quantum dot: 2-10 nm diameter, 100-100,000 atoms). The size of the fluorophore might sterically hinder the tagged molecule, and affect the fluorescence polarity.