What is the role of HIF 1 alpha?
What is the role of HIF 1 alpha?
HIF1A overexpression is heavily implicated in promoting tumor growth and metastasis through its role in initiating angiogenesis and regulating cellular metabolism to overcome hypoxia. Hypoxia promotes apoptosis in both normal and tumor cells.
What does HIF protein do?
Hypoxia-Inducible Factor (HIF)-1 is a dimeric protein complex that plays an integral role in the body’s response to low oxygen concentrations, or hypoxia. HIF-1 is among the primary genes involved in the homeostatic process, which can increase vascularization in hypoxic areas such as localized ischemia and tumors.
How many HIF target genes are there?
(A) There are over 70 known direct HIF target genes that function in numerous aspects of both normal physiology and disease processes, including angiogenesis and oxygen supply, stemness/self-renewal, proliferation, epithelial to mesenchymal transition (EMT), metastasis and invasion, redox homeostasis and apoptosis.
Which are considered targets of the transcription factor HIF 1α?
The known hypoxia-inducible factor (HIF) targets such as erythropoietin (EPO), vascular endothelial growth factor (VEGF), heme oxygenase-1 (HO-1), adrenomedullin (ADM), glucose transporter-1 (Glut-1), etc., exert neuroprotective effects on the central nervous system (CNS; Harten et al., 2010).
What is the function of the transcription factor HIF 2a?
HIF-1a functionally antagonizes c-Myc transcriptional activity under hypoxia, leading to upregulation of the cyclin-dependent kinase inhibitor p21 and cell cycle arrest. 64 In contrast, HIF- 2a promotes cell cycle progression by enhancing c-Myc- mediated expression of cyclin D2 and E2F1 in multiple cell types.
What is the role of prolyl hydroxylase?
The prolyl hydroxylase domain (PHD) enzymes regulate the stability of the hypoxia-inducible factor (HIF) in response to oxygen availability. During oxygen limitation, the inhibition of PHD permits the stabilization of HIF, allowing the cellular adaptation to hypoxia.
Can HIF-1 alpha be used to quantify the amount of protein?
However, it does not allow you to quantify the amount of protein in the sample. If you need to quantify protein, we recommend lysing your cells in RIPA buffer containing protease inhibitors. Because HIF-1 alpha is rapidly degraded in normoxia conditions, we recommend using HIF-1 stabilizers to inhibit degradation.
Does HIF-1 alpha have a transactivation domain?
Structural analysis of HIF-1 alpha revealed that dimerization with HIF-1 beta (ARNT) requires the HLH and PAS domains, DNA binding is mediated by the basic domain, and that HIF-1 alpha contains a carboxyl-terminal transactivation domain.
How do you stabilize HIF-1 alpha?
DMOG stabilizes HIF-1 alpha expression at concentrations between 0.1–1 mmol/L. Under hypoxic conditions, HIF-1 alpha is stabilized and translocates to the nucleus. Detection of HIF-1 alpha using nuclear lysates will increase the signal. Figure 1. Example blot. Ramos cells (Burkitt’s lymphoma cell line) were treated with CoCl2.
What is the role of HIF1 alpha in hypoxia?
HIF-1 alpha is a major global regulator of hypoxic response, and it is rapidly degraded in oxygenated cells. Degradation can occur within the first five minutes of exposure to normoxia, even after cells have been growing for long period in hypoxia.