Common questions

What is the size in kDa of the protein that is orange colored?

What is the size in kDa of the protein that is orange colored?

The 30 kDa band is red, 50 kDa band is green, 270 kDa band is orange, and other bands are blue. Broad Multi Color Pre-Stained Protein Standard is designed for observing protein separation during SDS-PAGE, verifying western transfer efficiency on membranes, and approximating the size of proteins.

How do you store a protein ladder?

Storage Note: To maximize shelf-life, the Prestained Protein Ladder should be boiled for 3–5 minutes upon receipt and aliquotted into single-use tubes. Store at –20°C. Guaranteed stable for 1 year when properly stored. Invitrogen Novex 10–20% Tris-glycine SDS-PAGE gel.

Do you need to boil protein ladder?

Do not boil the protein ladder. In high-percentage gels (14-18 %), large proteins (150-200 kDa) may not separate. In low-percentage gels (4-8 %), low-molecular weight proteins may migrate with the dye front.

What is unstained protein ladder?

Unstained Protein Ladder is a mixture of 12 recombinant, highly purified proteins, which resolve into clearly identifiable sharp bands from 10-250 kDa when analyzed by SDS-PAGE and stained with Coomassie Brilliant Blue R-250 (1,2). This ladder is intended for use as a precise size standard…

What is a broad range protein ladder?

Prestained Protein Ladder, Broad Range (10-230 kDa) is a mixture of highly purified recombinant proteins covalently coupled to a blue dye that resolves into 12 sharp bands when electrophoresed. The protein concentrations are carefully balanced for even intensity.

What is the KDA of unstained protein?

Unstained Protein Standard, Broad Range (10-200 kDa) The Unstained Protein Standard, Broad Range is a mixture of 12 recombinant, highly purified proteins, which resolve into clearly identifiable sharp bands from 10-200 kDa when analyzed by SDS-PAGE and stained with a commercially available, ready-to-use protein stain.

Can I order prestained protein ladder for overnight delivery?

Place your order before 7:30pm EST for overnight delivery. For shipping questions click here. Prestained Protein Ladder, Broad Range (10-230 kDa) is a mixture of highly purified recombinant proteins covalently coupled to a blue dye that resolves into 12 sharp bands when electrophoresed.