What is the use of Westergren tube?

ESR tubes measure the rate at which red blood cells settle out of plasma. Westergren sedimentation rate tubes may be used for the standard procedure, by drawing blood in a liquid sodium citrate vacuum tube, or the modified procedure, using a saline-diluted EDTA vacuum tube.

What is Westergren rack?

Westergren method: The blood is drawn into a Westergren-Katz tube to the 200 mm mark. The tube is placed in a rack in a strictly vertical position for 1 hour at room temperature, at which time the distance from the lowest point of the surface meniscus to the upper limit of the red cell sediment is measured.

What is the Westergren test?

Description. Erythrocyte sedimentation rate (ESR) westergren is an easy, inexpensive, nonspecific test that has been used for many years to help diagnose conditions associated with acute and chronic inflammation, including infections, cancers, and autoimmune diseases.

What is the modified Westergren method?

In the modified Westergren method for determining erythrocyte sedimentation rate (ESR), anticoagulated blood is diluted with 0.85% saline and aspirated into a calibrated tube. 1,2,3,4. The cells are allowed to settle for a period of exactly one hour.

Why is Westergren reference method?

The objective of ESR testing is to measure inflammability state. The Westergren method measures both infections related to infectious diseases and chronic inflammatory conditions.

Which is better Wintrobe or Westergren?

Usually, the Westergren method is preferred over the Wintrobe. But different articles give different reasons. Important factors involved are anticoagulant used, length of tubes used, external environmental pressure, extent of readings, the term used sensitivity and specificity, sometimes accuracy also used.

What is CBC in blood test?

Overview. A complete blood count (CBC) is a blood test used to evaluate your overall health and detect a wide range of disorders, including anemia, infection and leukemia. A complete blood count test measures several components and features of your blood, including: Red blood cells, which carry oxygen.

How do you read ESR in Westergren tube?

Procedure for ESR estimation: Fill the westergren tube up to mark 0 and place in the rack at room temperature undisturbed and away from sunlight. Take the reading exactly after 1 hour. Record in millimeters from top surface of column to top of RBC sediments.

Which ESR method is best?

The International Council for Standardization in Haematology recommended the Westergren method as the method of choice. The Westergren method uses citrate, a liquid-based anticoagulant, resulting in dilution of blood, inaccuracies of which significantly affect the ESR.

How do you measure the PCV of a Wintrobe tube?

After balancing the opposite bucket, centrifuge the tubes for 30 minutes at 2000 3000 rpm. Remove the tubes and carefully place it in the Wintrobe tube stand. Measure the height of the RBCs layer in mm. The obtained figure is the packed cell volume and is expressed in percentage.

How do you use a Westergren tube?

Fill the westergren tube up to mark 0 and place in the rack at room temperature undisturbed and away from sunlight. Take the reading exactly after 1 hour. Record in millimeters from top surface of column to top of RBC sediments.

What is the difference between the Westergren and Westergren methods?

Each method produces slightly different results. Most laboratories use the Westergren method. Westergren method: The Westergren method requires collecting 2 ml of venous blood into a tube containing 0 .5 ml of sodium citrate. It should be stored no longer than 2 hours at room temperature or 6 hours at 4 °C.

How is the Westergren-Katz test performed?

The blood is drawn into a Westergren-Katz tube to the 200 mm mark. The tube is placed in a rack in a strictly vertical position for 1 hour at room temperature, at which time the distance from the lowest point of the surface meniscus to the upper limit of the red cell sediment is measured.

What is the difference between Westergren’s stand and Wintrobe stand?

Markings from 0-100 mm from upto down are used for reading ESR and markings from bottom to upward used for reading PCV (hematocrit). Wintrobe’s stand: It is a small stand as compared to Westergren’s stand. lt is used to keep the Wintrobe’s tube vertically. The left sided series are used for ESR with O at the top.